BIO

My name is Taylor and I am from Saint Petersburg, Florida. I am currently a sophomore majoring in Public health with a minor in chemistry. I plan on attending medical school after I graduate and specializing in oncology. I have always been interested in research related to cancer as I have had a family member who has passed away from cancer. In my free time, I like to read, workout, and hangout with friends and family.

Lentivirus Transduction on MIA PaCa-2 Cells

Abstract

Lentivirus transduction is a set of protocols used to implement a desired gene into one's cells of interest. Lentivirus system is composed of two methods known as transfection and transduction. In the case of this project, transfection and transduction were used to introduce the LMNA gene into MIA PaCa-2 cells. This was completed as a method of validation of our CRISPRa/i system in the cells. Comprehending these various protocols along with others and why they are used is essential in understanding other methods used in a laboratory. These techniques also allow us to alter gene expression and study our genes of interest.

Keywords: Lentivirus, Cancer, CRISPR