Overall Research Project Description The exploration of three-dimensional (3D) cell culture models and the potential applications of nanoparticle-based therapies has emerged as a promising platform for the cancer research. Traditional two-dimensional (2D) cell culture models often fail to accurately mimic the complex microenvironment of solid tumors, which limits their translational relevance. However, 3D cell culture systems offer a more physiologically relevant platform to study cancer progression and therapeutic interventions. The primary objective of this project is to investigate the effects of different nanoparticle treatments on cancer cells using a 3D cell culture model. By utilizing a 3D culture system, the project aims to better simulate the intricate interactions between cancer cells, extracellular matrix components, and neighboring cells found within tumor microenvironments. The study will focus on understanding how various nanoparticles can influence cancer cell growth, invasion, and response to treatment in a more accurate representation of in vivo conditions.