Research Mentor Information
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Primary Research Mentor Name: Terence S Crofts
Research Mentor Preferred Pronouns: he/him
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Contact Email (FSU Email if affiliated): tcrofts@fsu.edu
Position Title: Faculty
FSU College (if applicable): Medicine
FSU Department or Non-FSU Organization Affiliation: Florida State University
Headshot (optional): https://cre.fsu.edu/system/files/webform/urop_project_proposal_portal/8411/220331_CroftsLogo_v2.jpg

Additional Research Mentor(s)
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Research Assistant Supervisor (if different from above): Ezabelle Franck
Research Assistant Supervisor Preferred Pronouns: she/her
Research Assistant Supervisor Preferred Honorific?: {Empty}
Contact Email (FSU Email if affiliated): efranck@fsu.edu
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Other Faculty/Collaborator(s) Preferred Pronouns: {Empty}
Other Faculty/Collaborator(s) Preferred Honorific?: {Empty}
Contact Email (FSU Email if affiliated): {Empty}

Overall Project Details
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Title of the Project: Novel antibiotic resistance genes from the environment
Project Keywords: antibiotic, resistance, bacteria, cloning, microbiology
Are you currently looking for research assistants?: No
Number of Research Assistants Needed : 2
Relevant Research Assistant Major(s):
Open to all majors

Project Location:: On FSU Main Campus
If the project location is off campus, does the research assistant(s) need to provide their own transportation?: {Empty}
Please select the choice that most accurately describes your expectations for the research assistant(s):: In-person
Approximately how many hours a week would the research assistant(s) need to work?: 10
Roughly what time frame do you expect research assistant(s) to work?: During business hours
Overall Research Project Description:
UROP students will be helping with projects studying the genes and mechanisms underlying bacterial resistance to antibiotics or other antimicrobials. This will involve identifying and cloning target genes, expressing them in E. coli, and measuring changes in E. coli antimicrobial susceptibility as a result. UROP researchers will work with a more senior undergraduate student to learn techniques and to help move projects forward.

Research Tasks:
Gene annotation and analysis
Buffer and media preparation
Polymerase chain reaction, cloning, agarose gel electrophoresis
E. coli culturing and transformation
Antimicrobial susceptibility testing

Skills that research assistant(s) may need::
Required
Previous work with micropipettes in a lab or class
Some MCB, microbiology, chemistry, etc lab exposure
Recommended
Microbiology lab or MCB lab
Microbiology or MCB classwork

Mentoring Philosophy:
We are a microbiology research group consisting of Dr. Terence Crofts (PI), Ezabelle Franck (lab manager), Hayden Allman (technician), and Elizabeth Bernate  
 and Sophia Vizoso (undergraduate researchers). Motivated UROP students will receive training to conduct supervised research into new antibiotic resistance genes with the goal of generating preliminary data that may expand into future projects. Committed students who work well as part of a team on a shared project are particularly encouraged to apply.

Please provide a link to your publications, a video clip, or a website for your research project (if applicable):: https://www.croftsmicrolab.org/
Please add any additional information here (if applicable)::
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Roundtable times and Zoom links:
Thursday September 7th from 2 pm to 3 pm
https://fsu.zoom.us/j/8416257485
Meeting ID: 841 625 7485

UROP Program Elements
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Mentor Handbook, FAQs, and Communication: Yes
UROP Performance Evaluation: Yes
Materials Grant: Yes
UROP Poster Presentation: Yes

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Year: 2023
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