Research Symposium

BIO

Sabrina was raised in Cúcuta, Colombia. Her favorite subject is chemistry and she hopes to become a geriatric doctor in the future.

NADPH Oxidase-derived ROS Effects on Lipolysis and Cardiometabolic Diseases

Abstract

Background: Previous research has found a link between a high concentration of reactive oxygen species (ROS) and endothelial dysfunction. In addition, evidence suggests that high ROS production contributes to dysregulated lipolysis, which is a common feature of obesity that is linked to impaired vascular function and insulin resistance. The purpose of this study is to examine a major source of ROS production, NADPH Oxidase (NOX), as a regulating factor of lipolysis and how the effects of Nox on lipolysis impact vascular function and glucose metabolism.

Methods: Young, healthy males and females completed a health screening to evaluate vitals (blood pressure and blood sugar) and body mass index. Cardiovascular fitness (a VO2max test) was completed by examining the maximum levels of oxygen utilized during exercise, as cardiovascular fitness levels are correlated with cardiometabolic health. A non-invasive ultrasound test was utilized to assess endothelial function of the brachial artery. A body composition (DXA) scan was performed to obtain data on lean mass and fat mass. Microdialysis was utilized to monitor local fat metabolism and ROS production in the subcutaneous abdominal adipose tissue before and during a hyperinsulinemic-euglycemic clamp procedure. During microdialysis, glycerol is measured as a marker of lipolysis and the contribution of NOX on total ROS production is measured via perfusion of a local NOX inhibitor (apocynin) into microdialysis probes.

We are collecting and analyzing data in our laboratory to determine the relationships between Nox and lipolysis, including how Nox-mediated lipolysis influences endothelial function and glucose metabolism.

Keywords: NADPH oxidase, Lipolysis, Cardio-metabolic diseases.