Research Symposium

BIO

Hi! My name is Sophia Vizoso and I am from Miami, Florida. I was raised by a very Cuban family and grew up hearing all about "the American Dream." Now that I am in college, I know that you can achieve anything you put yourself up to. I am currently a biochemistry major on the Pre-med track, and I hope to one day become a doctor. I have always been fascinated by the little things that move and grow but we can only see through the lens of a microscope. While at FSU, I've had the opportunity of working alongside Dr. Crofts, who has not only facilitated my learning, but has also increased my passion for the subject. Researching about antibiotic resistance and working with e. Coli has been one of the most unique and enriching opportunities in my life! So a special thanks to Dr. Crofts, Ezabelle Franck, and Elizabeth Bernate for allowing me to research alongside you all.

Tiamulin vs. E. coli: who would win?

Abstract

Over the past few decades, the increase in antibiotic resistance has been nothing short from alarming. This study specifically investigates Tiamulin, an antibiotic from the mulin family. Research shows that after a functional metagenomic selection and the sequencing of resistant colonies, some mystery genes were found to give resistance. Although E. coli doesn’t typically show much interest in Tiamulin, it has been found that with an efflux inhibitor, they stop its growth. The goal in this study is to PCR amplify the mystery genes, clone them into a plasmid and put them in E. coli, and lastly measure E. coli tiamulin resistance.

Keywords: Antibiotic, microbiology, genes, science, bacteria