BIO

Hello, I'm Ana Rodriguez Santos, with roots in Cuba and a heart shaped by Miami. Currently studying cell and molecular neuroscience on the pre-med journey, my passion lies in unraveling the mysteries of biochemistry, particularly delving into protein structures using NMR.

Purification of LA-Related Protein 7 for Biophysical Characterization

Abstract

LARP-6 is an RNA binding protein that is responsible for the regulation of type I collagen. LARP-6 can regulate collagen production through post-transcriptional regulation by binding to the mRNA before translation takes place. In Fibrotic diseases, a buildup of collagen is observed in the body. Here we used recombinant protein expression to produce LARP-6. We joined together the DNA code of t-RNA and the DNA code of LARP-6 in a plasmid and using PCR we expressed many copies to study its interaction with the mRNA. This research used different techniques such as Nuclear Magnetic Resonance and Cryogenic electron microscopy to observe how LARP-6 and RNA bind together. Understanding how LARP-6 and RNA bind together to control collagen production can help us understand how to prevent the buildup of collagen in people with fibrotic diseases.

Keywords: NMR, LARP-7