Research Symposium

BIO

Hello, my name is Sarah Bass and I am a sophomore at FSU. I am from the Chicago area, and my research interests include genomics and other biological/medical areas. This is my second year presenting at the Undergraduate Research Symposium, and it has been a great experience!

The Search for Mitotic DNA Synthesis (MiDAS) in Maize Root Tips

Abstract

This on-going study uses 3D epifluorescence imaging to understand the implications of Mitotic DNA synthesis (MiDAS), a rare and elusive abnormality within DNA synthesis. The study uses both primary and secondary (seminal) root tips of Zea mays strains to create multi-nucleic slides that were then observed using fluorescent staining techniques. The Leica Thunder Imaging System then captured 3-D, multi-wavelength (DAPI for DNA, FITC for DNA synthesis) image files that were uploaded to OMERO server (omero.bio.fsu.edu) for visual analysis. Data was derived through observations and quantified by distinguishing mitotic cells from other nuclei. The stains were then cross referenced with each other to detect MiDAS within the mitotic cells. Overall, the study processed imagery gained from multi-nucleic slides to detect and determine MiDAS within root tips of Zea mays strains. The results so far showcase MiDAS as a rare occurrence in maize root tips.

Keywords: Maize, Genomics, Mitosis