Research Symposium

BIO

I am a transfer student, and this is currently my final semester of my undergraduate studies. I am interested in 3D microscopy, as well as understanding more about molecular biology. I also plan on attending graduate school at FSU for the Cell and Molecular Biology Doctoral Program. I would like to one day be a lab manager in a government facility or become a college professor, maybe both!

The Search for Mitotic DNA Synthesis (MiDAS) in Maize Root Tips

Abstract

This ongoing study uses 3D epifluorescence imaging to understand the implications of Mitotic DNA synthesis (MiDAS), a rare and elusive abnormality within DNA synthesis. The study uses both primary and secondary (seminal) root tips of maize (Zea mays L.) strains to create multi-nucleic slides that were then observed using fluorescent staining techniques. The Leica Thunder Imaging System then captured 3-D, multi-wavelength (DAPI for DNA, FITC for DNA synthesis) image files that were uploaded to OMERO server (omero.bio.fsu.edu) for visual analysis. Data was derived through observations and quantified by distinguishing mitotic cells from other nuclei. The stains were then cross referenced with each other to detect MiDAS within the mitotic cells. Overall, the study processed imagery gained from multi-nucleic slides to detect and determine MiDAS within root tips of maize. The results so far showcase MiDAS as a rare occurrence in maize root tips.

Keywords: Microscopy, Genetics, Nuclei