BIO

My name is Yvonne, and I am currently a sophomore majoring in cell and molecular neuroscience. I have always been fascinated by the complexities of biological systems and their potential applications in improving human health. Beyond my current research endeavors, I aspire to pursue a career in medicine as a pediatrician.

Synthesis of S-Adenosylmethionine Using Escherichia coli SAM Synthetase

Abstract

S-adenosylmethionine (SAM) is an important molecule that participates in many biological processes, such as antibiotic biosynthesis in bacteria.1 The commercially available SAM usually contains 20-30% impurity. This is because SAM isomerizes from (R,S)-configuration to (S,S)-configuration, only the former of which is biologically active. In this study, Escherichia coli BL21(DE3) was used to overexpress SAM synthetase, which was then used to synthesize SAM in vitro.2 SAM synthetase acts as a biocatalyst, facilitating the chemical reaction between methionine and ATP. I adapted and optimized the protocol of SAM synthesis and analysis. My research offers insights into enhancing the efficiency of SAM production, thereby fostering the development of novel antibiotics in the future.

Keywords: Biochemistry, Synthesis, E.coli, Synthetase