UROP Research Mentor Project Submission Portal: Submission #428

Submission information
Submission Number: 428
Submission ID: 8411
Submission UUID: 7e7a9b26-0134-457a-8522-024acf0abbd3

Created: Sun, 08/13/2023 - 03:01 PM
Completed: Sun, 08/13/2023 - 03:25 PM
Changed: Mon, 09/25/2023 - 02:54 PM

Remote IP address: 217.180.192.224
Submitted by: Anonymous
Language: English

Is draft: No

Research Mentor Information

Terence S Crofts
he/him
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tcrofts@fsu.edu
Faculty
Medicine
Florida State University
220331_CroftsLogo_v2.jpg

Additional Research Mentor(s)

Ezabelle Franck
she/her
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efranck@fsu.edu
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Overall Project Details

Novel antibiotic resistance genes from the environment
antibiotic, resistance, bacteria, cloning, microbiology
No
2
Open to all majors
On FSU Main Campus
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In-person
10
During business hours
UROP students will be helping with projects studying the genes and mechanisms underlying bacterial resistance to antibiotics or other antimicrobials. This will involve identifying and cloning target genes, expressing them in E. coli, and measuring changes in E. coli antimicrobial susceptibility as a result. UROP researchers will work with a more senior undergraduate student to learn techniques and to help move projects forward.
Gene annotation and analysis
Buffer and media preparation
Polymerase chain reaction, cloning, agarose gel electrophoresis
E. coli culturing and transformation
Antimicrobial susceptibility testing
Required
Previous work with micropipettes in a lab or class
Some MCB, microbiology, chemistry, etc lab exposure
Recommended
Microbiology lab or MCB lab
Microbiology or MCB classwork
We are a microbiology research group consisting of Dr. Terence Crofts (PI), Ezabelle Franck (lab manager), Hayden Allman (technician), and Elizabeth Bernate
and Sophia Vizoso (undergraduate researchers). Motivated UROP students will receive training to conduct supervised research into new antibiotic resistance genes with the goal of generating preliminary data that may expand into future projects. Committed students who work well as part of a team on a shared project are particularly encouraged to apply.
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UROP Program Elements

Yes
Yes
Yes
Yes
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2023
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