UROP Research Mentor Project Submission Portal: Submission #421

Submission information
Submission Number: 421
Submission ID: 8376
Submission UUID: f8c88334-3f81-48ce-89cc-9307c839ffe1

Created: Fri, 08/11/2023 - 03:05 PM
Completed: Fri, 08/11/2023 - 03:05 PM
Changed: Mon, 09/25/2023 - 12:10 PM

Remote IP address: 144.174.212.19
Submitted by: Anonymous
Language: English

Is draft: No

Research Mentor Information

Nolan Blackford
He, Him
Mr.
ndb13b@fsu.edu
Graduate Student
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Arts and Sciences
Chemistry and Biochemistry
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Additional Research Mentor(s)

Robert Silvers
He, Him
Dr.
rsilvers@fsu.edu
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Overall Project Details

The Structural Characterization of Lupus Antigen-Related Proteins with Solution-State NMR
Biochemistry, Laboratory, Chemistry, Nuclear Magnetic Resonance, Structural Biology
No
2
Chemistry, Biology or Biochemistry
On FSU Main Campus
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In-person
~8
Flexible schedule (Combination of business and outside of business. TBD between student and research mentor.)
Our research is focused on the characterization of RNA binding proteins by utilizing a variety of biochemical techniques with emphasis on Nuclear Magnetic Resonance (NMR). With this project specifically, we aim to elucidate the structure and function of a series of RNA bind proteins known as the Lupus Antigen-Related proteins or LARPs. Students assisting with this project will be exposed to a multitude of biochemical techniques involved in the purification and characterization of recombinant proteins including but not limited to: bacterial transformation and expression, liquid chromatography (HPLC and FPLC), gel electrophoresis, and NMR.
Working with pipettes and laboratory glassware, Using machines such as centrifuges, shakers and incubators, Handling both liquid and solid chemicals. some data processing using Microsoft Excel and/or specialized software, working with and growing bacteria, learning to use specialized instruments.
Required: Ability to calculate concentrations and pH of buffer solutions. Recommended: Basic experience with laboratory glassware (undergraduate chemistry lab courses are sufficient)
As a mentor, my goal is to promote creativity and problem solving skills while sharing a passion for the subject matter. Providing challenges in a supportive environment can help boost confidence as well as teach students what they are really capable of. I want students to feel welcome and comfortable, without the work becoming too mundane or stagnant. As a result, I aim to continuously provide new challenges and opportunities to learn, but at a smooth and steady pace to prevent the work from becoming too daunting or stressful. This, I feel, is the best environment for personal growth. Entering academia in the hard sciences, in my experience, can be extremally intimidating as there are so many incredibly knowledgeable and skilled people. My goal is to clear the path between undergraduate and graduate study and give mentees the skills to make the transition smoothly and with confidence.
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UROP Program Elements

Yes
Yes
Yes
Yes
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No
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2023
https://cre.fsu.edu/urop-research-mentor-project-submission-portal?token=XyiR5N4bKzjFzNC0G2wEvuQ5buhKYVn-OJaAWqI2qdI